Abstract
Genetically modified soybeans were processed into finished, refined, bleached, and deodorized oils. Fatty acid composition was determined by gas-liquid chromatography. Glyceride structure was characterized according to degree of unsaturation by high-performance liquid chromatography, lipase hydrolysis, and gas-liquid chromatography. Compared to common varieties with 15% saturated acids, genetically modified soybeans yielded oils containing 24–40% saturated acids. Several varieties were examined, including the Pioneer A-90, Hartz HS-1, and Iowa State A-6 lines. Pioneer A-90 contained 17% stearic acid, had a solid fat index (SFI) of 6.0 at 10°C (50°F) and zero from 21.1 to 40°C (70 to 104°F), and therefore lacked sufficient solids for tub-type margarine. To improve its plastic range, the Pioneer oil was blended with palm oil, randomized palm oil, or interesterified palm/soy trisaturate basestock. After blending with 10–40% of these components, the high-stearic acid oil had an SFI profile suitable for soft tube margarine. The A-6 varieties, 32–38% saturates, showed SFI profiles with sufficient solids at 10°C (50°F) and 21.1°C (70°F) to qualify as a stick-type margarine oil, but lacked sufficient solids at 33.3°C (92°F); however, after small amounts (2–3%) of cottonseed or soybean hardstocks were added, the A-6 oils qualified as stick margarine oil. The HS-1 variety, when blended with small amounts (2–3%) of hardstock, possessed sufficient solids at 10°–33.3°C (50–92°F) to prepare soft tub margarine oil.
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References
Mounts, T.L., K. Warner, G.R. List, W.E. Neff, and R.F. Wilson, Low-Linolenic Acid Soybean Oils-Alternatives to Hydrogenated Cooking Oils,J. Am. Oil Chem. Soc. 71:495–499 (1994).
Mounts, T.L., K. Warner, and G.R. List, Performance Evaluation of Hexane Extracted Oils from Genetically Modified Soybeans,1:157–161 (1994).
Hammond, E.G., and W.R. Fehr, Registration of A-5 Germ Plasm Line of Soybean,Crop Sci. 23:192 (1983).
Wilson, R.F., and J.W. Bulton, Effects of Growth Temperature on Expression of a High Stearic Acid Trait in Soybean, inProceedings World Conference on Oilseed Technology and Utilization, American Oil Chemists' Society, Champaign, 1992, pp. 422–429.
Graef, G.L., L.A. Miller, W.R. Fehr, and E.G. Hammond, Fatty Acid Development in a Soybean Mutant with High Stearic Acid,J. Am. Oil Chem. Soc. 62:773–775 (1985).
List, G.R., T.L. Mounts, F. Orthoefer, and W.E. Neff, Margarine and Shortening Oils by Interesterification of Liquid and Trisaturated Triglycerides,72:379–382 (1995).
Neff, W.E., R.O. Adlof, G.R. List, and M. El-Agaimy, Analysis of Vegetable Oil Triacylglycerols by Silver Ion High-Performance Liquid Chromatography with Flame-Ionization Detection,J. Liq. Chromatogr., 17:3951–3968 (1994).
Official Methods and Recommended Practices of the American Oil Chemists' Society, edited by D. Firestone, American Oil Chemists' Society, Champaign, 1989, Methods Cd 10-57, Cc 18–80.
Neff, W.E., M.A.M. Zeitoun, and D. Weisleder, Resolution of Lipolysis Mixtures from Soybean Oil by a Solid Phase Extraction Procddure,J. of Chromatogr. 589:353–357 (1992).
List, G.R., E.A. Emken, W.F. Kwolek, T.P. Simpson, and H.J. Dutton, Preparation, Structure, and Properties of Interesterified Soybean Oil Soybean Trisaturate Blends,J. Am. Oil Chem. Soc. 54:408–413 (1977).
Duns, M.L., Palm Oil in Margarines and Shortenings,62:408–410 (1985).
Bessler, T.R., and F.T. Orthoefer, Providing Lubricity in Food Fat System,60:1765–1768 (1983).
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List, G.R., Mounts, T.L., Orthoefer, F. et al. Potential margarine oils from genetically modified soybeans. J Am Oil Chem Soc 73, 729–732 (1996). https://doi.org/10.1007/BF02517948
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DOI: https://doi.org/10.1007/BF02517948